Qualitative Differences Between Conscious and Nonconscious Processing? On Inverse Priming Induced by Masked Arrows.

In general, both consciously and unconsciously perceived stimuli facilitate responses to following similar stimuli. However, masked arrows delay responses to following arrows. This inverse priming has been ascribed to inhibition of premature motor activation, more recently even to special processing of nonconsciously perceived material. Here, inverse priming depended on particular masks, was insensitive to contextual requirements for increased inhibition, and was constant across response speeds. Putative signs of motor inhibition in the electroencephalogram may as well reflect activation of the opposite response. Consequently, rather than profiting from inhibition of primed responses, the alternative response is directly primed by perceptual interactions of primes and masks. Thus there is no need to assume separate pathways for nonconscious and conscious processing. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

The W-Process for Software Product Evaluation: A Method for Goal-Oriented Implementation of the ISO 14598 Standard

The importance of software product evaluations will grow with the awareness of the need for better software quality. The process to conduct such evaluations is crucial to get evaluation results that can be applied and meet customers' expectations. This paper reviews a well-known evaluation process: the ISO 14598 standard. The review focuses on the difficulties in selecting and evaluating the appropriate evaluation techniques. The review shows that the standard has problems in applying evaluation processes in practice due to insufficient attention to goal definition and to relationships between activities being implicit. Also, the standard ignores the trade-off between goals and resources and pays insufficient attention to feedback. To address these deficiencies, the W-process is proposed. It extends the standard through an improved process structure and additional guidelines.     

Glutathione-S-transferase M1 regulation of diesel exhaust particle-induced pro-inflammatory mediator expression in normal human bronchial epithelial cells

ABSTRACT: BACKGROUND: Diesel exhaust particles (DEP) contribute substantially to ambient particulate matter (PM) air pollution in urban areas. Inhalation of PM has been associated with increased incidence of lung disease in susceptible populations. We have demonstrated that the glutathione Stransferase M1 (GSTM1) null genotype could aggravate DEP-induced airway inflammation in human subjects. Given the critical role airway epithelial cells play in the pathogenesis of airway inflammation, we established the GSTM1 deficiency condition in primary bronchial epithelial cells from human volunteers with GSTM1 sufficient genotype (GSTM1+) using GSTM1 shRNA to determine whether GSTM1 deficiency could exaggerate DEP-induced expression of interleukin-8 (IL-8) and IL-1beta proteins. Furthermore, the mechanisms underlying GSTM1 regulation of DEP-induced IL-8 and IL-1beta expression were also investigated. METHODS: IL-8 and IL-1beta protein levels were measured using enzyme-linked immunosorbent assay. GSTM1 deficiency in primary human bronchial epithelial cells was achieved using lentiviral GSTM1 shRNA particles and verified using real-time polymerase chain reaction and immunoblotting. Intracellular reactive oxygen species (ROS) production was evaluated using flow cytometry. Phosphorylation of protein kinases was detected using immunoblotting. RESULTS: Exposure of primary human bronchial epithelial cells (GSTM1+) to 25-100 mug/ml DEP for 24 h significantly increased IL-8 and IL-1beta protein expression. Knockdown of GSTM1 in these cells further elevated DEP-induced IL-8 and IL-1beta expression, implying that GSTM1 deficiency aggravated DEP-induced pro-inflammatory response. DEP stimulation induced the phosphorylation of extracellular signal-regulated kinase (ERK) and Akt, the downstream kinase of phosphoinositide 3-kinase (PI3K), in GSTM1+ bronchial epithelial cells. Pharmacological inhibition of ERK kinase and PI3K activity blocked DEP-induced IL-8 and IL-1beta expression. DEP-induced ERK and Akt phosphorylation could be increased by GSTM1 knockdown. In addition, pretreatment of HBEC with the antioxidant N-acetyl cysteine significantly inhibited DEP-induced ERK and Akt phosphorylation, and subsequent IL-8 and IL-beta expression. CONCLUSION: GSTM1 regulates DEP-induced IL-8 and IL-1beta expression in primary human bronchial epithelial cells by modulation of ROS, ERK and Akt signaling.     

Development and evaluation of an intervention to reduce rip current related beach drowning

The objective of this research was to evaluate a campaign to improve beachgoer recognition of calm-looking rip currents, known to contribute to surf drowning. Posters, postcards, and brochures conveying the message “Don’t get sucked in by the rip” were distributed in an intervention area. Beachgoers were interviewed in this and a similar control area one year before and immediately after the intervention (respective response rates: 69.9% and 82.3%), Consenting respondents were sent follow-up questionnaires after approximately 6 months and 55% responded. In the intervention area, 28.8% of post-intervention, and 57.2% of follow-up respondents, had seen our campaign. At post-intervention, intervention respondents demonstrated improvement (relative to baseline) in intentions to swim away from a calm-looking rip, ability and confidence in identifying a rip, intention never to swim at unpatrolled beaches, and responses to being caught in a rip, compared to the control respondents. Similar improvements were observed post-intervention for respondents in the intervention area who had seen our campaign (relative to those who had not), and at 6 month follow-up for intervention respondents (relative to control respondents). The relatively brief print-based campaign was effective in warning beachgoers about calm-looking rips.     

Persistent UAV Service: An Improved Scheduling Formulation and Prototypes of System Components

The flight duration of unmanned aerial vehicles (UAVs) is limited by their battery or fuel capacity. As a consequence, the duration of missions that can be pursued by UAVs without supporting logistics is restricted. However, a system of UAVs that is supported by automated logistics structures, such as fuel service stations and orchestration algorithms, may pursue missions of conceivably indefinite duration. This may be accomplished by handing off the mission tasks to fully fueled replacement UAVs when the current fleet grows weary. The drained UAVs then seek replenishment from nearby logistics support facilities. To support the vision of a persistent fleet of UAVs pursuing missions across a field of operations, we develop an improved mixed integer linear programming (MILP) model that can serve to support the system’s efforts to orchestrate the operations of numerous UAVs, missions and logistics facilities. Further, we look toward the future implementation of such a persistent fleet outdoors and develop prototype components required for such a system. In particular, we develop and demonstrate the concerted operation of a scheduling model, UAV onboard vision-based guidance system and replenishment stations.     

Tablet PC-based Visual Target-Following System for Quadrotors

This paper proposes a vision-based, closed-loop target-following system for quadrotors. The system consists of a vision-based target detection algorithm that uses the color and image moment of a given target. Flight control commands are directly generated based on the offset of the target from the image frame center. The image processing and control algorithms have been implemented on a latest tablet computer, which is capable of running those algorithms in real time. The proposed system was demonstrated using a commercially available quadrotor platform equipped with a forward-facing camera. Experiments and their analyses showed satisfactory target following performance.     

An Artificial Nocturnal Flower via Humidity‐Gated Photoactuation in Liquid Crystal Networks

Beyond their colorful appearances and versatile geometries, flowers can self‐shape‐morph by adapting to environmental changes. Such responses are often regulated by a delicate interplay between different stimuli such as temperature, light, and humidity, giving rise to the beauty and complexity of the plant kingdom. Nature inspires scientists to realize artificial systems that mimic their natural counterparts in function, flexibility, and adaptation. Yet, many of the artificial systems demonstrated to date fail to mimic the adaptive functions, due to the lack of multi‐responsivity and sophisticated control over deformation directionality. Herein, a new class of liquid‐crystal‐network (LCN) photoactuators whose response is controlled by delicate interplay between light and humidity is presented. Using a novel deformation mechanism in LCNs, humidity‐gated photoactuation, an artificial nocturnal flower is devised that is closed under daylight conditions when the humidity level is low and/or the light level is high, while it opens in the dark when the humidity level is high. The humidity‐gated photoactuators can be fueled with lower light intensities than conventional photothermal LCN actuators. This, combined with facile control over the speed, geometry, and directionality of movements, renders the “nocturnal actuator” promising for smart and adaptive bioinspired microrobotics.